BactoReal® Kit Listeria monocytogenes

Unit: 50 or 100

Order number: DVEB008xx

For further information please contact us here

 

BactoReal Kit Listeria monocytogenesEppi Kit DNA

 

 

BactoReal®  Kit Listeria monocytogenes

Order no.

Reactions

Pathogen

Internal positive control

DVEB00813

100

FAM channel

Cy5 channel

DVEB00853

50

FAM channel

Cy5 channel

DVEB00811

100

FAM channel

VIC/HEX channel

DVEB00851

50

FAM channel

VIC/HEX channel

Kit content:
Detection assay for Listeria monocytogenes
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for L. monocytogenes
Water

Background: Listeriosis is an infection caused by eating food (milk, cheeses, ice cream, vegetables, sausages, meats and fish) contaminated with the bacterium called Listeria monocytogenes. Although there are other types of Listeria (L. ivanovii, L. innocua, L. welshimeri, L. seegligeri and L. grayi), most cases of listeriosis are caused by┬áL. monocytogenes. Listeria are ubiquitously found in soil and water. Gastrointestinal symptoms may precede more serious forms of listeriosis or may be the only symptoms expressed. The two main severe clinical manifestations of listeriosis are sepsis and meningitis. People at highest risk include the elderly, the fetuses of pregnant women, and the immunosuppressed. Listeria monocytogenes is a gram positive, facultative anaerobic, and facultative intracellular bacterium. Its incubation time is at least seven days. ItÔÇÖs ability to grow at temperatures as low as 0┬░C permits multiplication in refrigerated foods. Listeria monocytogenes is killed by pasteurization and cooking. It expresses a Beta hemolysin (hlyA gene) causing destruction of red blood cells.

Description: BactoReal® Kit Listeria monocytogenes is based on the amplification and detection of the hlyA gene of  L. monocytogenes using real-time PCR.

PCR-platforms: BactoReal® Kit Listeria monocytogenes is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Listeria monocytogenes


Inge
netix ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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