Plant Real

 

 

The Plant Real kit is designed to quantify DNA from vegetal species. The assay targets a segment of the chloroplast DNA and was designed to detect vegetal species used in food production or animal feed but can be also used for other applications.
The Plant Real kit detects plant species, such as maize (Zea mays), rapeseed (Brassica napus), barley (Hordeum vulgare), common oat (Avena sativa), rice (Oryza sativa), common wheat (Triticum aestivum), rye (Secale cereale), soybean (Glycine max), sunflower (Helianthus annuus), dinkel wheat or spelt (Triticum aestivum subsp. spelta), peanut (Arachis hypogaea), almond (Prunus dulcis), pumpkin (Cucurbita maxima), chickpea (Cicer arietinum) and lentil (Lens culinaris). The assay further detects common hazel (Corylus avellana), common walnut (Juglans regia), pecan (Carya illinoinensis), and Cashew nut (Anacardium occidentale). Performance of the assay with these plant species was verified experimentally.

Product Plant Real
Order no. RTGL100
Unit 100 reactions
Technology Real-time PCR
Kit Content Assay
Positive Control
Manual ABI PRISM® 7500
Target Gene Chloroplast-DNA
Detection 530 nm (FAM channel)
Validated PCR platform Additionally required PCR reagents
ABI PRISM® 7500 TaqMan® Universal PCR Master Mix or TaqMan® Gene Expression Master Mix

Test was validated for ABI PRISM® 7500 and LightCycler® 1.1/1.2/1.5/2.0, but can also be used with other real-time PCR instruments which detect and differentiate fluorescence at 530 nm (FAM channel). These are e.g. LightCycler® 480, ABI PRISM® 7000/7300/7700/7900, StepOnePlus™, Rotor-Gene™ 3000/6000, iCycler iQ™, iQ™5, CFX96™/CFX384™, Opticon® 2, Chromo4™, Mastercycler® ep realplex, SmartCycler/SmartCycler® II, MX3000P/MX3005P/MX4000®, etc.

For further information please see product description RTGL100

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