BactoReal® Listeria monocytogenes

 

BactoReal Listeria monocytogenes_RTGV

 

Background: Listeriosis is an infection caused by eating food (milk, cheeses, ice cream, vegetables, sausages, meats and fish) contaminated with the bacterium called Listeria monocytogenes. Although there are other types of Listeria (L. ivanovii, L. innocua, L. welshimeri, L. seegligeri and L. grayi), most cases of listeriosis are caused by L. monocytogenes. Listeria are ubiquitously found in soil and water. Gastrointestinal symptoms may precede more serious forms of listeriosis or may be the only symptoms expressed. The two main severe clinical manifestations of listeriosis are sepsis and meningitis. People at highest risk include the elderly, the fetuses of pregnant women, and the immunosuppressed. Listeria monocytogenes is a gram positive, facultative anaerobic, and facultative intracellular bacterium. Its incubation time is at least seven days. It’s ability to grow at temperatures as low as 0°C permits multiplication in refrigerated foods. Listeria monocytogenes is killed by pasteurization and cooking. It expresses a Beta hemolysin (hlyA gene) causing destruction of red blood cells.

BactoReal® Listeria monocytogenes is a detection assay for DNA of Listeria monocytogenes by real-time polymerase chain reaction.

Also available as kitversion: BactoReal® Kit Listeria monocytogenes

Product BactoReal® Listeria monocytogenes
Order no. RTGV1200
Unit 100 reactions
Technology Real-time PCR
Target gene hly gene, detection in FAM channel
Content Assay (pimers + probe) for Listeria monocytogenes
Positive Control Listeria monocytogenes
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.5/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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