BactoReal® Bartonella spp.

 

BactoReal Bartonella_RTGM

 

Background: Bartonella species are gram-negative, fastidious bacteria. In addition to dogs and cats, numerous domestic and wild animals, including bovine, canine, and rodent species as well as humans can serve as a reservoir host for various Bartonella species. Cats may harbor B. henselae, B. clarridgeiae and rarely other Bartonella species. Dogs may carry Bartonella vinsonii subsp. berkhoffii, Bartonella henselae and others. Humans can be a reservoir or an incidental host for B. bacilliformis, B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. rochalimae, B. vinsonii and B. washoensis. Bartonella bacilliformis causes Carrion’s disease (Oroya fever and Verruga peruana). Bartonella henselae, which frequently causes chronic bacteremia in cats, is the most common etiologic agent of cat scratch disease. It can also cause bacillary angiomatosis, peliosis hepatitis, and sometimes endocarditis in immunocompromised human patients. Bartonella clarridgeiae can cause chronic bacteremia in cats and has been associated with cat scratch disease in humans. Bartonella quintana is the etiologic agent of trench fever and has also been associated with bacteremia, endocarditis, and bacillary angiomatosis. The other Bartonella species infecting humans have mainly been implicated in endocarditis and neuroretinitis.

BactoReal® Bartonella spp. is a detection assay for rapid and sensitive detection of DNA of B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis by real-time PCR.
Bartonella rochalimae, B. vinsonii and B. bovis are detected with very low sensitivity. Bartonella bacilliformis is not detected.

Product BactoReal® Bartonella spp.
Order no. RTGM905
Unit 50 reactions
CE-IVD No
Technology Real-time PCR
Target gene gltA gene, detection in FAM channel
Content Assay (primers + probe) for Bartonella
Positive Control Bartonella henselae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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