BactoReal® Bordetella bronchiseptica


BactoReal Bordetella bronchiseptica_RTGM


Background: Bordetella bronchiseptica is a gram-negative bacterium found as a normal inhabitant of the respiratory tract in a variety of animals such as dogs, pigs and some rodents. In some cases it causes pneumonia, canine infectious tracheobronchitis (kennel cough), bronchopneumonia in cats, and atrophic rhinitis in pigs. Transmission can occur through direct contact with respiratory secretions, fomites, or inhalation of infected aerosol. In humans, it can cause infectious bronchitis.

BactoReal® Bordetella bronchiseptica is a detection assay for rapid and sensitive detection of DNA of Bordetella bronchiseptica by real-time PCR.

Product BactoReal® Bordetella bronchiseptica
Order no. RTGM1450
Unit 50 reactions
Technology Real-time PCR
Target gene bfra gene, detection in FAM channel
Content Assay (pimers + probe) for B. bronchiseptica
Positive Control B. bronchiseptica
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.