BactoReal® Bordetella parapertussis


BactoReal Bordetella parapertussis 


Background: Bordetella parapertussis is a Gram-negative bacterium of the genus Bordetella. Transmission occurs through close contact with contaminated respiratory droplets. While B. pertussis is the etiologic agent of whooping cough, B. parapertussis, B. holmesii and B. bronchiseptica may cause a pertussis-like respiratory disease but with a milder clinical course. Bordetella pertussis and B. parapertussis are exclusively human pathogens, whereas B. bronchiseptica most often infects animals, but is infrequently isolated also from humans.

BactoReal® Bordetella parapertussis is a detection assay for rapid and sensitive detection of DNA of Bordetella parapertussis by real-time PCR. The test was designed to detect also some strains of B. bronchiseptica and B. holmesii. An additional analysis with the BactoReal® Bordetella pertussis (ingenetix order no. RTGM1500) can largely differ between an infection with B. parapertussis or B. holmesii / B. bronchiseptica.

Product BactoReal® Bordetella parapertussis
Order no. RTGM1400
Unit 50 reactions
Technology Real-time PCR
Target gene IS1001 region, detection in FAM channel
Content Assay (pimers + probe) for B. parapertussis
Positive Control B. parapertussis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.