BactoReal® Bordetella pertussis


BactoReal Bordetella pertussis


Background: Bordetella pertussis is a Gram-negative bacterium of the genus Bordetella and the etiologic agent of whooping cough, an acute respiratory disease. Transmission occurs through close contact with contaminated respiratory droplets. A pertussis-like respiratory disease but with a milder clinical course may be caused by B. parapertussis, B. holmesii and B. bronchiseptica. While B. pertussis and B. parapertussis are exclusively human pathogens, B. bronchiseptica most often infects animals, but is infrequently isolated also from humans.

BactoReal® Bordetella pertussis is a detection assay for rapid and sensitive detection of DNA of Bordetella pertussis by real-time PCR. The test was designed to detect also B. holmesii and some strains of B. bronchiseptica. An additional analysis with BactoReal® Bordetella parapertussis (ingenetix order no. RTGM1400) can largely differ between an infection with B. pertussis or B. holmesii / B. bronchiseptica.

Product BactoReal® Bordetella pertussis
Order no. RTGM1500
Unit 50 reactions
Technology Real-time PCR
Target gene IS481 region, detection in FAM channel
Content Assay (pimers + probe) for B. pertussis
Positive Control B. pertussis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.