BactoReal® Borrelia burgdorferi sensu lato


 BactoReal Borrelia_RTGM


Background: Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause the Lyme disease (Lyme borreliosis). Theses species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. The Lyme disease is a multisystemic illness which can affect various organs, especially the nervous system, skin, heart and joints. Other human pathogenic Borrelia species are B. recurrentis, B. duttonii and B. hermsii, causing relapsing fever.

BactoReal® Borrelia burgdorferi sensu lato (ospA) is a detection assay for rapid and sensitive detection of DNA of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex by real-time PCR. Borrelia spielmanii is detected with a decreasecd sensitivity by this test.

Also available as CE-IVD certified kitversion: BactoReal® Kit Borrelia burgdorferi sensu lato

Product BactoReal® Borrelia burgdorferi sensu lato
Order no. RTGM500
Unit 50 reactions
Technology Real-time PCR
Target gene ospA gene, detection in FAM channel
Content Assay (pimers + probe) for Borrelia burgdorferi sensu lato
Positive Control Borrelia
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.