BactoReal® Brucella spp.

 

BactoReal Brucella spp_RTGM

 

Background: Brucellosis – also called Bang’s disease, Crimean fever, Gibraltar fever, Malta fever, Maltese fever, Mediterranean fever, rock fever, or undulant fever – is a zoonotic infection of domesticated and wild animals, caused by bacteria of the genus Brucella. Humans become infected by ingestion of food products of animal origin (such as undercooked meat or unpasteurized milk or dairy products), direct contact with infected animals, or inhalation of infectious aerosols. Transmission from human to human is rare but possible. Species infecting domestic livestock are B. melitensis (goats and sheep), B. suis (pigs), B. abortus (cattle and bison), B. ovis (sheep) and B. canis (dogs).

BactoReal® Brucella spp. is a detection assay for rapid and sensitive detection of DNA of B. abortus, B. ovis, B. melitensis, B. suis and B. canis by real-time PCR.

Also available as kitversion: BactoReal® Kit Brucella spp.

Product BactoReal® Brucella spp.
Order no. RTGM1150
Unit 50 reactions
CE-IVD No
Technology Real-time PCR
Target gene IS711 region, detection in FAM channel
Content Assay (pimers + probe) for Brucella spp.
Positive Control Brucella abortus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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