BactoReal® Clostridium difficile

 

BactoReal Clostridium difficile_RTGM

 

Background: Clostridium difficile is a gram-positive spore-forming anaerobic bacterium which is commonly found in low concentrations in the intestine of clinically normal animals and humans. Pathogenic C. difficile strains produce multiple toxins. The best characterized are enterotoxin (Clostridium difficile toxin A) and cytotoxin (Clostridium difficile toxin B), both of which may produce diarrhea and inflammation. Clostridium difficile infection (CDI) is a major cause of nosocomial antibiotic-associated infectious diarrhea and pseudomembranous colitis in humans.

BactoReal® Clostridium difficile is a detection assay for rapid and sensitive detection of the Clostridium difficile toxin A (tcdA) gene and Clostridium difficile toxin B (tcdB) gene by multiplex real-time PCR.

Product BactoReal® Clostridium difficile
Order no. RTGM1270
Unit 50 reactions
CE-IVD No
Technology Real-time PCR
Target gene tcdA gene, detection in VIC/HEX channel
tcdB gene, detection in FAM channel
Content Assay (primers + probe) for tcda and tcdB
Positive Control tcda and tcdB
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real 3 (order no. RTGMCR-3) contains primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assay 3 (order no. RTGMIPC-3) contains primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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