BactoReal® Haemophilus influenzae


BactoReal Haemophilus influenzae


Background: The 16 species of Haemophilus are Gram-negative bacteria occasionally colonizing the mucosa of humans and animals. Some Haemophilus species are the causative agents of various infections. Haemophilus influenza can be found exclusively in the mucosa of humans, mainly in the upper respiratory tract. Transmission occurs through close contact with contaminated respiratory droplets. If encapsulated, H. influenzae is an obligate pathogenic bacterium. In sum, six capsular serotypes (a-f) can be distinguished, out of which type b (Hib) is the most prevalent and virulent one causing bloodstream infections and meningitis mainly in children less than 5 years. In contrast, non-capsulated strains (also called nontypeable strains) of H. influenzae are part of the normal bacterial flora of humans and are occasionally pathogen. These strains can cause otitis, sinusitis, epiglottitis, bronchitis, pneumonia and less frequently meningitis and bacteraemia. The Omp P6 gene of H. influenzae is present in both the encapsulated and non-capsulated strains of H. influenzae.

BactoReal® Haemophilus influenzae is a detection assay for rapid and sensitive detection of DNA of encapsulated and non-capsulated Haemophilus influenzae strains by real-time PCR.

Also available as CE-IVD certified kitversion: BactoReal® Kit Haemophilus influenzae

Product BactoReal® Haemophilus influenzae
Order no. RTGM300
Unit 50 reactions
Technology Real-time PCR
Target gene ompP6 gene, detection in FAM channel
Content Assay (pimers + probe) for H. influenzae
Positive Control H. influenzae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.