BactoReal® Legionella pneumophila


BactoReal Legionella pneumophila


Background: Legionella are Gram-negative bacteria frequently found in aquatic systems and living within amoebae. The most common devices that have been found to transmit Legionella are domestic hot-water systems heated to less than 70┬░C, air conditioners and cooling towers. Legionella are transmitted to the lungs of human beings through aerosolized water. No person-to-person transmission has been yet observed. Many different species of Legionella (at least 40) have been discovered, and more than 66 serogroups of Legionella have been identified. Many thereof are considered to be potentially pathogenic for humans. Infection by Legionella pneumophila results mainly in Legionnaire’s disease. However, some strains manifest a disorder called Pontiac Fever, which is a milder infection than the Legionnaire’s disease.

BactoReal® Legionella pneumophila is a detection assay for rapid and sensitive detection of DNA of Legionella pneumophila by real-time PCR. This test detects all known serogroups and subspecies of L. pneumophila. Other Legionella species occasionally causing pneumonia are not detected.

Also available as CE-IVD certified kitversion: BactoReal® Kit Legionella pneumophila

Product BactoReal® Legionella pneumophila
Order no. RTGM1600
Unit 50 reactions
Technology Real-time PCR
Target gene 23S-5S intergenic region, detection in FAM channel
Content Assay (pimers + probe) for L. pneumophila
Positive Control L. pneumophila
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.