BactoReal® Leptospira spp. (16S rDNA)


BactoReal Leptospira 16S_RTGMBakterien Assay Vet Vic


Background: The spirochaetal Leptospira genus consists of pathogenic species, intermediately pathogenic species and of non-pathogenic species. Members of Leptospira can also be grouped into serovars. Currently over 200 serovars are recognized in the genus Leptospira and a few serovars are found in more than one species of Leptospira. Non-pathogenic species are saprophytes and can grow outside the host animal, while pathogenic Leptospira species cause leptospirosis. They affect many mammalian species, including humans. Animals may become unapparent carriers, and shedding of leptospires, primarily in the urine, serves as a source of infection for other animals and humans. The clinical signs associated with leptospirosis are variable and depend on the infecting serovar and the susceptibility of the animal. Leptospires are present in the blood during the first 5 to 10 days after onset of the disease.

BactoReal® Leptospira spp. (16S rDNA) is a detection assay for DNA of pathogenic and intermediately pathogenic Leptospira species by real-time polymerase chain reaction.

Product BactoReal® Leptospira spp. (16S rDNA)
Order no. RTGM1700
Unit 50 reactions
Technology Real-time PCR
Target gene 16S rRNA gene, detection in VIC/HEX channel
Content Assay (pimers + probe) for Leptospira
Positive Control Leptospira
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real 3 (order no. RTGMCR-3) contains primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assay 3 (order no. RTGMIPC-3) contains primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.