BactoReal® Listeria monocytogenes


BactoReal Listeria monocytogenes_RTGM


Background: Listeriosis is an infection caused by eating food (milk, cheeses, ice cream, vegetables, sausages, meats and fish) contaminated with the bacterium called Listeria monocytogenes. Although there are other types of Listeria (L. ivanovii, L. innocua, L. welshimeri, L. seegligeri and L. grayi), most cases of listeriosis are caused by L. monocytogenes. Listeria are ubiquitously found in soil and water. Gastrointestinal symptoms may precede more serious forms of listeriosis or may be the only symptoms expressed. The two main severe clinical manifestations of listeriosis are sepsis and meningitis. People at highest risk include the elderly, the fetuses of pregnant women, and the immunosuppressed. Listeria monocytogenes is a gram positive, facultative anaerobic, and facultative intracellular bacterium. Its incubation time is at least seven days. It’s ability to grow at temperatures as low as 0°C permits multiplication in refrigerated foods. Listeria monocytogenes is killed by pasteurization and cooking.

BactoReal® Listeria monocytogenes is a detection assay for rapid and sensitive detection of DNA of Listeria monocytogenes by real-time PCR.

Also available as CE-IVD certified kitversion: BactoReal® Kit Listeria monocytogenes

Product BactoReal® Listeria monocytogenes
Order no. RTGM200
Unit 50 reactions
Technology Real-time PCR
Target gene hlyA gene, detection in FAM channel
Content Assay (primers + probe) for L. monocytogenes
Positive Control L. monocytogenes
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.