BactoReal® Mycoplasma pneumoniae


BactoReal Mycoplasma pneumoniae


Background: Mycoplasma pneumoniae is a very small bacterium lacking a cell wall. This bacterium has a global distribution. Mycoplasma pneumoniae is an obligate pathogen and highly infectious. Transmission occurs through close contact with contaminated respiratory droplets. Mycoplasma pneumoniae infects the mucosal membrane of the respiratory tract and is a common cause of both upper and lower respiratory infections, including tracheobronchitis and primary atypical pneumonia (community-acquired pneumonia, CAP), but it can also cause pharyngitis, meningitis and otitis media.

BactoReal® Mycoplasma pneumoniae is a detection assay for rapid and sensitive detection of DNA of Mycoplasma pneumoniae by real-time PCR.

Also available as CE-IVD certified kitversion: BactoReal® Kit Mycoplasma pneumoniae

Product BactoReal® Mycoplasma pneumoniae
Order no. RTGM1300
Unit 50 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in FAM channel
Content Assay (primers + probe) for M. pneumoniae
Positive Control M. pneumoniae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.