BactoReal® Streptococcus pneumoniae


BactoReal Streptococcus pneumoniae


Background: Streptococcus pneumoniae (Pneumococci) is a Gram-positive, alpha-hemolytic bacterium which can cause pneumonia (community-acquired pneumonia, CAP), bronchitis, otitis media, meningitis and sepsis. Pneumococci commonly colonize the upper human respiratory tract and can also be found in healthy persons (40% und 70%). Transmission occurs through close contact with contaminated respiratory droplets. The risk of a pneumococcal infection is much increased in persons with a weak immune system.

BactoReal® Streptococcus pneumoniae is a detection assay for rapid and sensitive detection of DNA of S. pneumoniae by real-time PCR. The test is specific for S. pneumoniae.

Also available as CE-IVD certified kitversion: BactoReal® Kit Streptococcus pneumoniae

Product BactoReal® Streptococcus pneumoniae
Order no. RTGM601
Unit 50 reactions
Technology Real-time PCR
Target gene lytA gene, detection in FAM channel
Content Assay (primers + probe) for S. pneumoniae
Positive Control S. pneumoniae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.