BactoReal® Tropheryma whipplei


BactoReal Tropheryma whippleiRTGM grün DNA


Background: Tropheryma whipplei is a gram-postive bacterium that it is very difficult to cultivate. It is a human pathogen causing Whipple’s disease, which is a rare, chronic, systemic illness typically affecting white, middle-aged men. It causes intestinal malabsorption that leads to cachexia. In the majority of cases, multiple organ systems are affected. Symptoms include diarrhea and abdominal pain, joint pain, arthritis, and even dementia. It manifests in the body in various forms and can go undetected for decades before suddenly causing a fatal illness.

BactoReal® Tropheryma whipplei is a detection assay for rapid and sensitive detection Tropheryma whipplei DNA by real-time PCR.

Product BactoReal® Tropheryma whipplei
Order no. RTGM902
Unit 50 reactions
Technology Real-time PCR
Target gene groEL gene, detection in FAM channel
Content Assay (pimers + probe) for T. whipplei
Positive Control T. whipplei
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.