BactoReal® Chlamydophila psittaci/abortus

 

BactoReal Chlamydophila psiitaci_RTGM

 

Background: Chlamydophila psittaci (previously classified as Chlamydia psittaci) is an intracellular bacterium that may cause endemic avian chlamydiosis in feral birds and domesticated poultry, epizootic outbreaks in mammals such as cattle, pigs, sheep and horses, and respiratory psittacosis in humans (also known as parrot fever and ornithosis). It is transmitted by inhalation, contact or ingestion among birds and to mammals. Psittacosis in birds and in humans often starts with flu-like symptoms and becomes a life-threatening pneumonia. Chlamydophila psittaci is present in feces, nasal secretions, and feathers of infected birds. Chlamydophila abortus (previously classified as Chlamydia psittaci serotype 1) is one of the major infectious causes of abortion in sheep, cattle and goats worldwide by colonizing in the placenta. Enzootic abortion in sheep is also known as ovine enzootic abortion (OEA) or enzootic abortion of ewes (EAE). Infection with strains of this microorganism has also been associated with abortion and other clinical symptoms also in humans.

BactoReal® Chlamydophila psittaci/abortus is a detection assay for rapid and sensitive detection of DNA of Chlamydophila psittaci and C. abortus by real-time PCR.

Product BactoReal® Chlamydophila psittaci/abortus
Order no. RTGM1250
Unit 50 reactions
CE-IVD No
Technology Real-time PCR
Target gene omp1 gene, detection in FAM channel
Content Assay (pimers + probe) for C. psittaci+C. abortus
Positive Control C. psittaci
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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