BactoReal® Kit Haemophilus influenzae (CE-IVD)

 

BactoReal_Kit_DHUB_IVD_Heamophilus_influenzaeBactoReal_IVD_FAM

 

 

Background: The 16 species of Haemophilus are Gram-negative bacteria occasionally colonizing the mucosa of humans and animals. Some Haemophilus species are the causative agents of various infections. For example, H. aegyptius causes purulent conjunctivitis, H. parainfluenzae is a rare cause of a form of endocarditis. Haemophilus influenzae can be found exclusively in the mucosa of humans, mainly in the upper respiratory tract. Transmission occurs through close contact with contaminated respiratory droplets. In sum, six capsular serotypes (a-f) can be distinguished, out of which type b (Hib) is the most prevalent and virulent one causing bloodstream infections and meningitis mainly in children less than 5 years. In contrast, unencapsulated strains (also called nontypeable H. influenzae, NTHi) of H. influenzae are part of the normal bacterial flora of humans and are occasionally pathogen. These strains can cause otitis, sinusitis, epiglottitis, bronchitis, pneumonia and less frequently meningitis and bacteraemia. The Omp P6 gene of H. influenzae is present in both the encapsulated and unencapsulated strains of H. influenzae.

BactoReal® Kit Haemophilus influenzae is an in vitro diagnostic test, based on real-time PCR, for the detection of encapsulated and unencapsulated (also called nontypeable H. influenzae, NTHi) H. influenzae strains. The included internal DNA positive control system can be used as DNA extraction and PCR inhibition control.

Sensitivity and specificity: See manual or product description

Product BactoReal® Kit Haemophilus influenzae
Order no. DHUB01053
Unit 50 reactions
CE IVD Yes
Technology Real-time PCR
Target gene Omp P6 gene
Content + Assay (primer+probe, FAM) for H. influenzae
+ Assay (primer+probe, Cy5)+target for internal positive control system
+ DNA positive control for H. influenzae
+ DNA amplification mix
+ Nuclease-free water
PCR platform Tested with ABI PRISM® 7500, LightCycler® 480, MX3005P
Compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel

 

Manual v1.2e: Download pdf

Material Safety Data Sheet: Download pdf

Declaration of conformity: Download pdf

 

 


ViroReal®, BactoReal® und ParoReal Kits run under same thermal cycling conditions

RNA and DNA targets can be analysed in one run
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