BactoReal® Kit Bartonella spp.





Background: Bartonella species are gram-negative, fastidious bacteria. In addition to dogs and cats, numerous domestic and wild animals, including bovine, canine, and rodent species as well as humans can serve as a reservoir host for various Bartonella species. Cats may harbor B. henselae, B. clarridgeiae and rarely other Bartonella species. Dogs may carry Bartonella vinsonii subsp. berkhoffii, Bartonella henselae and others. Humans can be a reservoir or an incidental host for B. bacilliformis, B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. rochalimae, B. vinsonii and B. washoensis. Bartonella bacilliformis causes Carrion’s disease (Oroya fever and Verruga peruana). Bartonella henselae, which frequently causes chronic bacteremia in cats, is the most common etiologic agent of cat scratch disease. It can also cause bacillary angiomatosis, peliosis hepatitis, and sometimes endocarditis in immunocompromised human patients. Bartonella clarridgeiae can cause chronic bacteremia in cats and has been associated with cat scratch disease in humans. Bartonella quintana is the etiologic agent of trench fever and has also been associated with bacteremia, endocarditis, and bacillary angiomatosis. The other Bartonella species infecting humans have mainly been implicated in endocarditis and neuroretinitis.

BactoReal® Kit Bartonella spp. is a real-time PCR test for the detection of DNA of B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis.
Bartonella rochalimae, B. vinsonii and B. bovis are detected with very low sensitivity. Bartonella bacilliformis is not detected. The included internal DNA positive control system can be used as DNA extraction and PCR inhibition control.

Sensitivity and specificity: See manual or product description

Product BactoReal® Kit Bartonella spp.
Order no. DHUB03953
Unit 50 reactions
Technology Real-time PCR
Target gene gltA gene
Content + Assay (primer+probe, FAM) for Bartonella
+ Assay (primer+probe, Cy5)+target for internal positive control system
+ DNA positive control for B. henselae
+ DNA amplification mix
+ Nuclease-free water
PCR platform Tested with ABI PRISM® 7500, LightCycler® 480, MX3005P
Compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel


Manual v1.4en: Download pdf

Material Safety Data Sheet: Download pdf


ViroReal®, BactoReal® und ParoReal Kits run under same thermal cycling conditions

RNA and DNA targets can be analysed in one run