BactoReal® Kit Legionella pneumophila (CE-IVD)





Background: Legionella are Gram-negative bacteria frequently found in aquatic systems and living within amoebae. The most common devices that have been found to transmit Legionella are domestic hot-water systems heated to less than 70┬░C, air conditioners and cooling towers. Legionella are transmitted to the lungs of human beings through aerosolized water. No person-to-person transmission has been yet observed. Many different species of Legionella (at least 40) have been discovered, and more than 66 serogroups of Legionella have been identified. Many thereof are considered to be potentially pathogenic for humans. Infection by Legionella pneumophila results mainly in Legionnaire’s disease. However, some strains manifest a disorder called Pontiac Fever, which is a milder infection than the Legionnaire’s disease.

BactoReal® Kit Legionella pneumophila is an in vitro diagnostic test, based on real-time PCR, for the detection of Legionella pneumophila . The included internal DNA positive control system can be used as DNA extraction and PCR inhibition control.

Sensitivity and specificity: See manual or product description

Product BactoReal® Kit Legionella pneumophila
Order no. DHUB01153
Unit 50 reactions
Technology Real-time PCR
Target gene 23S-5S rDNA intergenic spacer region
Content + Assay (primer+probe, FAM) for L.  pneumophila
+ Assay (primer+probe, Cy5)+target for internal positive control system
+ DNA positive control for L. pneumophila
+ DNA amplification mix
+ Nuclease-free water
PCR platform Tested with ABI PRISM® 7500, LightCycler® 480, MX3005P
Compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel


Manual v1.2e: Download pdf

Material Safety Data Sheet: Download pdf

Declaration of conformity: Download pdf



ViroReal®, BactoReal® und ParoReal Kits run under same thermal cycling conditions

RNA and DNA targets can be analysed in one run