BactoReal® Kit Listeria monocytogenes (CE-IVD)





Background: Listeria are ubiquitously found in soil and water. Listeriosis is an infection caused by the consumption of contaminated food (milk, cheeses, ice cream, vegetables, sausages, meats and fish). Although there are other types of Listeria (L. ivanovii, L. innocua, L. welshimeri, L. seegligeri and L. grayi), the culprit pathogen for most listeriosis cases is L. monocytogenes. Gastrointestinal symptoms may remain the only symptoms or precede more severe clinical manifestations such as sepsis and meningitis. People at risk include the elderly population, immunosuppressed patients and foetuses of pregnant women. L. monocytogenes is a gram positive, facultative anaerobic, and facultative intracellular bacterium with an incubation time of at least seven days. It expresses a Beta hemolysin (hlyA gene) which causes destruction of red blood cells and has the ability to target other cells. The ability of L. monocytogenes to grow at temperatures as low as 0°C permits multiplication in refrigerated foods. L. monocytogenes is killed by pasteurization and cooking.

BactoReal® Kit Listeria monocytogenes is an in vitro diagnostic test, based on real-time PCR, for the detection of Listeria monocytogenes. The included internal DNA positive control system can be used as DNA extraction and PCR inhibition control.

Sensitivity and specificity: See manual or product description

Product BactoReal® Kit Listeria monocytogenes
Order no. DHUB00853
Unit 50 reactions
Technology Real-time PCR
Target gene hlyA gene
Content + Assay (primer+probe, FAM) for L. monocytogenes
+ Assay (primer+probe, Cy5)+target for internal positive control system
+ DNA positive control for L. monocytogenes
+ DNA amplification mix
+ Nuclease-free water
PCR platform Tested with ABI PRISM® 7500, LightCycler® 480, MX3005P
Compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel


Manual v1.0e: Download pdf

Material Safety Data Sheet: Download pdf

Declaration of conformity: Download pdf



ViroReal®, BactoReal® und ParoReal Kits run under same thermal cycling conditions

RNA and DNA targets can be analysed in one run