MycoReal Pneumocystis


MycoReal PneumocystisMcoReal Pneumocystis



Background: Pneumocystis jiroveci (formerly Pneumocystis carinii) is a yeast-like fungus which can be found worldwide. Pneumocystis jiroveci is a distinct species that only infects humans, while the related species P. carinii can be found in rodents and other mammals. Both healthy and immunocompromised people can be colonised with P. jiroveci. While it does not affect healthy people, P. jiroveci can cause an interstitial Pneumocystis-Pneumonia (PCP) in persons with HIV infection, with primary immune deficiencies, including hypogammaglobulinemia and severe combined immunodeficiency (SCID), persons receiving long-term immunosuppressive regimens for connective-tissue disorders, vasculitides, or solid-organ transplantation, persons with hematologic and nonhematologic malignancies, including solid tumors and lymphomas, and persons with severe malnutrition. Currently the diagnosis of PCP relies on microscopic methods or PCR, as P. jiroveci cannot be cultured in routine microbiology laboratories. Pneumocystis-PCR gains more and more importance in the diagnosis of a Pneumocystis-infection.

MycoReal Pneumocystis is developed and validated for the LightCycler® 1.2/1.5/2.0 and 480 instruments (Roche), the ABI PRISM® 7500 (Fast) instrument (Applied Biosystems), and Mx3005P® QPCR System (Agilent) but is also suitable for other real-time PCR instruments. Order number for the respective PCR-platforms see below.

Product MycoReal Pneumocystis
Unit 50 reactions
Technology Real-time PCR
Content Assay Pneumocystis Assay internal positive control+Internal positive control Positive control for Pneumocystis jiroveci Water Manual (for LightCycler® 1.2/1.5/2.0/480, ABI PRISM® 7500, MxPro®) Attention: Amplification mix is not included, see product description
Target gene Mt LSU

For use with the ABI PRISM® 7500 (Fast) instrument and the Mx3005P® QPCR System: MycoReal Pneumocystis order no. RTPM401 or RTPM403 P. jiroveci is detected in FAM channel (530 nm) and the internal positive control is detected in VIC/HEX channel or (order no. RTPM401) or in Cy5 channel (order no. RTPM403).

For use with the LightCycler® 480: MycoReal Pneumocystis order no. RTPM403 P. jiroveci is detected in FAM channel (530 nm) and the internal positive control is detected in Cy5 channel.

For use with the LightCycler® 1.2/1.5 or 2.0 instrument: MycoReal Pneumocystis order no. RTPM400 P. jiroveci is detected in channel F1 (530 nm) and the internal positive control is detected in channel F2 (610 nm). Since MycoReal Assays (MycoReal Aspergillus, MycoReal Candida, MycoReal Pneumocystis and MycoReal Fungi) use identical temperature/time profiles, they can be combined in the same LightCycler® 1.2/1.5/2.0 run. This allows the detection of a wide panel of pathogenic fungi within only a few hours.