ParoReal Acanthamoeba


ParoReal Acanthamoeba_RTGM BactoReal Assay Human FAM


Background: One of the six eukaryotic clusters comprise the Amoebozoa, of which the genera Acanthamoeba is primarily a free-living protozoan in natural habitats, but also causative agent of human and animal disease. Acanthamoeba serves as host for a variety of pathogenic bacteria such as Mycobacterium avium. Infestation with Acanthamoeba is associated with potentially sight-threatening contact lens-related keratitis, serious infections of other organs and fatal granulomatous amoebic encephalitis. More than 20 species of Acanthamoeba are known, which can be classified into three morphologic groups (Group I, II and III) and 15 genotypes (T1-T15).  Some species (A. astronyxis, A. byersi, A. castellanii, A. culbertsoni, A. divionensis, A. griffinii, A. hatchetti, A. healyii, A. lenticulata, A. lugdunensis, A. palestinensis, A. polyphaga, A. quina, A. rhysodes) have been recently associated with human disease. Genotype T4 has been considered the most important genotype in both ocular and CNS infections.

ParoReal Acanthamoeba is a detection assay for rapid and sensitive detection of DNA of Acanthamoeba by real-time PCR.

Also available as kitversion: ParoReal Kit Acanthamoeba

Product ParoReal Acanthamoeba
Order no. RTGM001P
Unit 50 reactions
Technology Real-time PCR
Target gene 18S rRNA gene, detection in FAM channel
Content Assay (primers + probe) for Acanthamoeba
Positive Control Acanthamoeba
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.