ViroReal® CMV


ViroReal CMVRTGM gr├╝n DNA


Background: Cytomegaly is a worldwide disease caused by the human cytomegalovirus (HCMV), also called human herpesvirus type 5 (HHV-5). HCMV is a double-stranded DNA virus transmissed by droplet and smear infection, breast feeding, blood components containing cells, as well as by stem cell- and organ transplantation. After infection HCMV persists in blood or the infected organs lifetime. Up to 80% of the healthy population are carriers of HCMV. For healthy adults HCMV infection is commonly asymptomatic or subclinical, while for persons with a weak immune system infection can lead to severe clinical pictures. Also, HCMV infection is the most common congenital viral infection and may be acquired prenatally or perinatally. For fetuses and newborns HCMV can cause life-threatening infections with possible long-term consequences.

ViroReal® CMV is a detection assay for rapid and sensitive detection of DNA of the human cytomegalovirus (HCMV) by real-time PCR.

Product ViroReal® CMV
Order no. RTGM10V
Unit 50 reactions
Technology Real-time PCR
Target gene UL44 gene, detection in FAM channel
Content Assay (primers + probe) for CMV
Positive Control CMV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

  BactoReal®, ParoReal and ViroReal® Assays detecting bacterial, parasitic and viral DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.