ViroReal® Enterovirus

 

ViroReal EnterovirusRTGM gr├╝n RNA

 

Background: Enteroviruses are non-enveloped, positive single-stranded RNA viruses (ss(+)RNA) and are members of the family of Picornaviridae. The genus Enterovirus has a worldwide distribution and includes numerous viral groups infecting human beings, bovines, equines, simians, porcines and rodents. Enteroviruses infecting humans are poliovirus, coxsackievirus, echovirus, human enteroviruses and human rhinoviruses. Enteroviruses are the causative agents e.g. for poliomyelitis, aseptic meningitis, herpangina, hand-foot-mouth disease, hemorrhagic conjunctivitis, infection of the respiratory tract (summer flu, pharyngitis, pneumonia) and infection of inner organs (myocarditis, encephalitis, pleurodynia).

ViroReal® Enterovirus is a detection assay for rapid and sensitive detection of RNA of the the genus Enterovirus by reverse transcription real-time PCR. Rhinoviruses are detected with less sensitivity by this test (see ViroReal® Rhinovirus).

Also available as CE-IVD certified kitversion: ViroReal® Kit Enterovirus

Product ViroReal® Enterovirus
Order no. RTGM20RV
Unit 50 reactions
CE-IVD No
Technology Reverse transcription real-time PCR
Target gene 5┬┤UTR region, detection in FAM channel
Content Assay (primers + probe) for Enterovirus
Positive Control Enterovirus
Amplification mix & IPC Not included, see product description
PCR-platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.5/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube (for control of RNA extraction and RT-PCR inhibition). See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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