ViroReal® HHV-6A, B


ViroReal HHV6AB


Background: Human herpes virus type 6 (HHV-6) is a double-stranded DNA virus which occurs as two variants (HHV-6 type A and HHV-6 type B). It is a ubiquitous virus infecting nearly 90% of the population before two years of age. The virus is transmissed by droplet infection. Primary infections in children may stay unapparent or can cause three-day fever (also called exanthema subitum, roseola infantum or sixth disease). The three-day fever is mainly due to HHV-6 type B, but can also be caused by the human herpes virus type 7 (HHV-7). HHV-6A is more neurovirulent. The rare primary infection in adults can cause hepatitis and mononucleosis-like disease. After infection HHV-6 persists in the organism. In immunocompromised hosts, HHV-6 reactivation can cause localized organ disease, organ rejection, encephalitis, pneumonia, hepatitis and leukopenia.

ViroReal® HHV-6A, B is a detection assay for rapid and sensitive detection of DNA of both types A and B of HHV-6 by real-time PCR.

For the differentiation of type 6A and type 6B ingenetix offers ViroReal® HHV-6A and ViroReal® HHV-6B.

Product ViroReal® HHV-6A, B
Order no. RTGM16V
Unit 50 reactions
Technology Real-time PCR
Target gene U65/U66 gene, detection in FAM channel
Content Assay (primers + probe) for HHV-6
Positive Control HHV-6
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.