ViroReal® Human Coronavirus alpha


ViroReal Human Coronavirus alpha



Background: Coronaviruses are positive single-stranded (+)ssRNA RNA viruses of the family Coronaviridae. Several different strains of coronaviruses are currently known to infect humans (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV, NcoV and HCoV-EMC). Strains HCoV-229E, HCoV-NL63, HCoV-OC43, MERS-CoV and HCoV-HKU1 cause cold, upper respiratory infection, bronchiolitis and pneumonia in humans. SARS-CoV causes the Severe Acute Respiratory Syndrome.

ViroReal® Human Coronavirus alpha is a detection assay for rapid and sensitive detection of RNA of human coronavirus NL63 and 229E  by multiplex reverse transcription  real-time PCR.

Product ViroReal® Human Coronavirus alpha
Order no. RTGM40RV
Unit 50 reactions
Technology Reverse transcription real-time PCR
Target gene N gene, detection in FAM and VIC/HEX channel
Content Assay (primers + probe) for coronavirus HCoV-229E (VIC probe) und HCoV-NL63 (FAM probe)
Positive Control HCoV-229E + HCoV-NL63
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.5/2.0/480 and MX3005P®. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) – not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR the Internal Positive RNA Control Assay 3 can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assay 3 (order no. RTGMIPCR3) contains primers, a probe and an internal control RNA target in an extra tube (for control of RNA extraction and RT-PCR inhibition). See product description.

ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.