ViroReal® Influenza B

 

ViroReal Influenza BRTGM gr├╝n RNA

 

Background: Influenza is an acute infectious disease caused by influenza virus A, B or, to a much lesser extent, influenza virus C. Influenza viruses are enveloped viruses with single-stranded, segmented RNA with negative polarity as genome. These viruses can be found worldwide. Epidemics and pandemics are mainly caused by influenza virus A, due to antigenic drift of the hemagglutinin and neuraminidase molecules. Type B and C influenza viruses are isolated almost exclusively from humans, while influenza A viruses infect a wide variety of warm-blooded animals.

ViroReal® Influenza B is a detection assay for rapid and sensitive detection of RNA of influenza B virus by reverse transcription real-time PCR.

Product ViroReal® Influenza B
Order no. RTGM16RV
Unit 50 reactions
CE-IVD No
Technology Reverse transcription real-time PCR
Target gene Hemagglutinin gene, detection in FAM channel
Content Assay (primers + probe) for influenza B
Positive Control influenza B
Amplification mix & IPC Not included, see product description
PCR-platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.5/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube (for control of RNA extraction and RT-PCR inhibition). See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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