ViroReal® Metapneumovirus

 

ViroReal MetapneumovirusRTGM gr├╝n RNA

 

Background: Human metapneumovirus (hMPV) is an enveloped negative single-stranded RNA virus (ss(-)RNA) of the family Paramyxoviridae. It was isolated for the first time in 2001. Human metapneumovirus can cause upper and lower respiratory tract infections. It has a worldwide distribution.

ViroReal® Metapneumovirus is a detection assay for rapid and sensitive detection of RNA of the human metapneumovirus by reverse transcription real-time PCR.

Also available as CE-IVD certified kitversion: ViroReal® Kit Metapneumovirus

Product ViroReal® Metapneumovirus
Order no. RTGM35RV
Unit 50 reactions
CE-IVD No
Technology Reverse transcription real-time PCR
Target gene Nucleocapsid protein gene, detection in FAM channel
Content Assay (primers + probe) for metapneumovirus
Positive Control metapneumovirus
Amplification mix & IPC Not included, see product description
PCR-platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.5/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube (for control of RNA extraction and RT-PCR inhibition). See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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