ViroReal® Parainfluenzavirus


RTGM gr├╝n RNA


Background: Human parainfluenzaviruses (HPIV) are enveloped, negative single-stranded RNA viruses (ss(-)RNA) of the family Paramyxoviridae. They can cause upper and lower respiratory tract infections (human parainfluenza). At present four serotypes are known (types 1, 2, 3, 4). Type 4 is rare and shows low clinical symptoms.

ViroReal® Parainfluenzavirus is a detection assay for rapid and sensitive detection of RNA of the human parainfluenzavirus types 1, 2, 3 and 4 by reverse transcription real-time PCR.

Available as CE-IVD certified kitversion: ViroReal® Kit Parainfluenzavirus

Product ViroReal® Parainfluenzavirus
Order no. RTGM34RV
Unit 50 reactions
Technology Reverse transcription real-time PCR
Target gene Hemagglutinin-neuraminidase glycoprotein gene, detection in FAM + VIC/HEX channel
Content Assay (primers + probe) for parainfluenzavirus types 1, 2, 3, 4
Positive Control Parainfluenzavirus 1, 2
Amplification mix & IPC Not included, see product description
PCR-platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay 3 can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assay 3 (order no. RTGMIPCR3) contains primers, a probe and an internal control RNA target in an extra tube (for control of RNA extraction and RT-PCR inhibition). See product description.

ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.