ViroReal® Parechovirus

 

ViroReal ParechovirusRTGM gr├╝n RNA

 

 

Background: Human parechoviruses are non-enveloped, positive single-stranded RNA viruses (ss(+)RNA) and are members of the family of Picornaviridae. Six serotypes of human parechovirus have been identified (human parechovirus 1-6). Parechoviruses are responsible for gastrointestinal, respiratory and central nervous system infections. 95% of humans are carriers of human parechovirus since early life.

ViroReal® Parechovirus is a detection assay for rapid and sensitive detection of RNA of the human parechovirus by reverse transcription real-time PCR.

Product ViroReal® Parechovirus
Order no. RTGM36RV
Unit 50 reactions
CE-IVD No
Technology Reverse transcription real-time PCR
Target gene Polyprotein gene, detection in FAM channel
Content Assay (primers + probe) for parechovirus
Positive Control parechovirus
Amplification mix & IPC Not included, see product description
PCR-platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.5/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube (for control of RNA extraction and RT-PCR inhibition). See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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