ViroReal® Parvovirus B19


ViroReal ParvovirusB19


Background: Parvovirus B19 is a linear single stranded DNA virus. The genetic diversity of the found B19 strains falls into the three well-recognized genotypes 1, 2 and 3 with approximately 10-15% nucleotide divergence between them. The virus is primarily spread by infected respiratory droplets, but also blood-borne or transplancentar transmission is possible. Infection with parvovirus B19 can cause erythema infectiosum, which is a childhood exanthem more commonly known as fifth disease, furthermore it might cause aplastic crisis, hydrops fetalis and arthropathy but 25% of cases can remain asymptomatic.

ViroReal® Parvovirus B19 is a detection assay for rapid and sensitive detection of DNA of the three genotypes (genotypes 1, 2 and 3) of parvovirus B19 by real-time PCR.

Product ViroReal® Parvovirus B19
Order no. RTGM19V
Unit 50 reactions
Technology Real-time PCR
Target gene VP1 gene, detection in FAM channel
Content Assay (primers + probe) for parvovirus B19
Positive Control parvovirus B19
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.