ViroReal® VZV


ViroReal VZV


Background: Varicella zoster virus (VZV), also called human herpes virus type 3 (HHV-3) is a double-stranded DNA virus. The VZV is found worldwide (> 95% of persons are seropositive) and is transmissed by droplet infection. Primary infection with VZV causes chickenpox. After primary infection, VZV remains dormant in sensory nerve roots for life. Upon reactivation, the virus migrates down the sensory nerve to the skin, causing herpes zoster (also called shingles). Shingles generally affects the elderly and people with an immune deficiency.

ViroReal® VZV is a detection assay for rapid and sensitive detection of DNA of the varicella zoster virus (VZV) by real-time PCR.

Product ViroReal® VZV
Order no. RTGM20V
Unit 50 reactions
Technology Real-time PCR
Target gene ORF29 region, detection in FAM channel
Content Assay (primers + probe) for VZV
Positive Control VZV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.