ViroReal® Kit Parainfluenzavirus (CE-IVD)





Background: Human parainfluenzaviruses (HPIV) are enveloped, negative single-stranded RNA viruses (ss(-)RNA) of the family Paramyxoviridae. At present four serotypes are known (types 1, 2, 3, 4). Parainfluenzavirus type 1 and 3 belong to the genus Respirovirus, while parainfluenzavirus type 2 and 4 belong to the genus Rubulavirus. Human parainfluenzavirus type 1 (HPIV-1) is the major cause of acute croup, but can also cause respiratory tract infections at all ages. Human parainfluenzavirus type 2 (HPIV-2) can also cause croup as well as upper and lower respiratory tract illnesses. Human parainfluenzavirus type 3 (HPIV-3) causes bronchiolitis and pneumonia. Human parainfluenzavirus type 4 with the subtypes 4a and 4b is less likely to cause servere disease.

ViroReal® Kit Parainfluenzavirus is an in vitro diagnostic test, based on one-step reverse transcription real-time PCR (RT-PCR), for the detection of parainfluenzavirus 1, 2, 3 and 4 strains (HPIV-1, HPIV-2, HPIV-3, HPIV-4). The included internal RNA positive control system can be used as RNA extraction and RT-PCR inhibition control.

Sensitivity and specificity: See manual or product description

Product ViroReal® Kit Parainfluenzavirus
Order no. DHUV01653
Unit 50 reactions
Technology One-step reverse transcription real-time PCR
Target gene HN gene
Content + Assay (primer+probe, FAM) for HPIV-1 and HPIV-3
+ Assay (primer+probe, VIC/HEX) for HPIV-2 and HPIV-4
+ Assay (primer+probe, Cy5)+target for internal positive control system
+ RNA positive control for HPIV-1 and HPIV-2
+ RNA amplification mix
+ Nuclease-free water
PCR platform Tested with ABI PRISM® 7500, LightCycler® 480, MX3005P
Compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM, VIC/HEX and Cy5 channel


Manual v1.2e: Download pdf

Material Safety Data Sheet: Download pdf

Declaration of conformity: Download pdf



ViroReal®, BactoReal® und ParoReal Kits run under same thermal cycling conditions

RNA and DNA targets can be analysed in one run