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Product Manual ViroReal® Pseudorabiesvirus (SHV-1) Download PDF
Material Safety Data Sheet ViroReal® Pseudorabiesvirus (SHV-1) Download PDF
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Background:  Pseudorabiesvirus (PRV) is a swine DNA herpesvirus of the Alphaherpesvirinae subfamily. It is also known as suid herpesvirus 1 (SHV-1), or by its original name, Aujeszky's disease virus. The pig is the principal reservoir host, but the virus may also infect other mammals like cattle, little ruminants, carnivores and rodents. It is not pathogenic for humans. In swine, Aujeszky’s disease shows itself under three forms: a nervous form, a respiratory form or a genital form. In latently infected pigs, pseudorabiesvirus persists primarily in the trigeminal or sacral ganglia and the tonsils. In other mammals, it causes infection of the central nervous system with neurologic behavioral changes leading very quickly to death.

Description:  ViroReal® Kit Pseudorabiesvirus uses real-time PCR for the detection of the glycoprotein B gene of SHV-1. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Pseudorabiesvirus (SHV-1)
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02513 100 FAM Cy5 glycoprotein B gene
DVEV02553 50 FAM Cy5 glycoprotein B gene
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: glycoprotein B gene of Pseudorabiesvirus (SHV-1)
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Pseudorabiesvirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Pseudorabiesvirus (SHV-1)
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV27 100 FAM Cy5 glycoprotein B gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.