Internal Positive RNA Control Assay 2

 

IPC RNA 2

 

 

Internal Positive RNA Control Assay 2 is developed for the LightCycler® 1.2/1.5 or 2.0 instruments (Roche), but can also be used with other real-time PCR instruments which detect fluorescence at 576 nm (NED channel). The internal positive RNA control target is added during extraction or to the mastermix. Thus, the amplification of the internal positive RNA control serves as control of RNA extraction and excludes false-negative interpretation of results due to real-time PCR-inhibition.
This assay can be combined with ViroReal® assays (see corresponding product descriptions) as well as with user-specific assays.

Product Internal Positive RNA Control Assay 2
Order no. RTGMIPCR2
Unit 100 reactions
CE-IVD No
Technology Reverse transcription real-time PCR
Content Assay (primer and probe) + internal positive RNA control target
Attention: Amplification mix is not included, see product description
Target gene Internal positive RNA control
Detection LightCycler® 1.2/1.5/2.0: Channel F2 or 610 nm
ABI PRISM® 7500 (Fast) Instrument : 576 nm (channel for NED)
Validated assays

See corresponding ViroReal® product descriptions

 

ingenetix real-time PCR control assays:

Information on detection channels and PCR-platforms   

1. ingenetix control assays for monotoring of PCR inhibition:
Assays are based on the amplification and detection of an internal positive DNA control target included in the primer-probe mix.
– ControlReal 1
– ControlReal 2
– ControlReal 3

2. ingenetix control assays for control of DNA extraction and monitoring of PCR inhibition:
Assays are based on the amplification and detection of an internal positive DNA control target added during extraction or to the mastermix.
Internal Positive Control Assay 1
Internal Positive Control Assay 2
Internal Positive Control Assay 3

3. ingenetix control assays for control of RNA extraction and monitoring of PCR inhibition:
Assays are based on the amplification and detection of an internal positive RNA control target added during extraction or to the mastermix.
Internal Positive RNA Control Assay 1
Internal Positive RNA Control Assay 2
Internal Positive RNA Control Assay 3

Information which control assay is suitable for your application can be found at the corresponding  BactoReal®, ViroReal® and ParoReal products.

Ingenetix real-time PCR control assays can also be combined with user-specific assays.

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