BactoReal® Kit Borrelia burgdorferi sensu lato

 

BactoReal Kit Borrelia

 

BactoReal® Kit Borrelia burgdorferi sensu lato
Order no. Reactions Pathogen

Internal positive control

DVEB05013

100

FAM channel

Cy5 channel

DVEB05053

50

FAM channel

Cy5 channel

DVEB05011

100

FAM channel

VIC/HEX channel

DVEB05051

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Borrelia burgdorferi sensu lato
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Borrelia
– Water

Background: Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause the Lyme disease (Lyme borreliosis). These species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. The Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

Description: BactoReal® Kit Borrelia burgdorferi sensu lato is based on the amplification and detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex using real-time PCR.

PCR-platforms: BactoReal® Kit Borrelia burgdorferi sensu lato is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Borrelia burgdorferi sensu lato


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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