BactoReal® Kit Brachyspira hyodysenteriae

 

BactoReal Kit Brachyspira hyodysenteriae

 

BactoReal®  Kit Brachyspira hyodysenteriae

Order no.

Reactions

Pathogen

Internal positive control

DVEB01113

100

FAM channel

Cy5 channel

DVEB01153

50

FAM channel

Cy5 channel

DVEB01111

100

FAM channel

VIC/HEX channel

DVEB01151

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for B. hyodysenteriae
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for B. hyodysenteriae
Water

Background: Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is an anaerobic intestinal spirochaete and the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira hyodysenteriae is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

Description: BactoReal® Kit Brachyspira hyodysenteriae is based on the amplification and detection of the nox gene of B. hyodysenteriae using real-time PCR.

PCR-platforms: BactoReal® Kit Brachyspira hyodysenteriae is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Brachyspira hyodysenteriae


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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