BactoReal® Kit Brachyspira hyo/pilo

 

BactoReal Kit Brachyspira hyo_piloBactoReal Kit FAM

 

BactoReal®  Kit Brachyspira hyo/pilo

Order no.

Reactions

Pathogen

Internal positive control

DVEB01813

100

FAM channel

Cy5 channel

DVEB01853

50

FAM channel

Cy5 channel

DVEB01811

100

FAM channel

VIC/HEX channel

DVEB01851

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for B. hyodysenteriae and B. pilosicoli
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive controls for B. hyodysenteriae and B. pilosicoli
Water

Background: Brachyspira are anaerobic intestinal spirochaetes. Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira pilosicoli (previously called Serpulina pilosicoli) colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS).

Description: BactoReal® Kit Brachyspira hyo/pilo is based on the amplification and detection of the nox gene of B. hyodysenteriae and B. pilosicoli using real-time PCR. Other Brachyspira species are not detected by this test.

PCR-platforms: BactoReal® Kit Brachyspira hyo/pilo is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Brachyspira hyo/pilo


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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