BactoReal® Kit Brachyspira pilosicoli

 

BactoReal Kit Brachyspira pilosicoli

 

BactoReal®  Kit Brachyspira pilosicoli

Order no.

Reactions

Pathogen

Internal positive control

DVEB01213

100

FAM channel

Cy5 channel

DVEB01253

50

FAM channel

Cy5 channel

DVEB01211

100

FAM channel

VIC/HEX channel

DVEB01251

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for B. pilosicoli
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for B. pilosicoli
Water

Background: Brachyspira pilosicoli (previously called Serpulina pilosicoli) is an anaerobic intestinal spirochaete. This bacterium colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS).

Description: BactoReal® Kit Brachyspira pilosicoli is based on the amplification and detection of the nox gene of B. pilosicoli using real-time PCR.

PCR-platforms: BactoReal® Kit Brachyspira pilosicoli is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Brachyspira pilosicoli


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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