BactoReal® Kit Brucella spp.

 

BactoReal Kit Brucella spp

 

BactoReal® Kit Brucella spp.
Order no. Reactions Pathogen

Internal positive control

DVEB02113

100

FAM channel

Cy5 channel

DVEB02153

50

FAM channel

Cy5 channel

DVEB02111

100

FAM channel

VIC/HEX channel

DVEB02151

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Brucella spp.
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for Brucella abortus
Water

Background: Brucellosis – also called Bang’s disease, Crimean fever, Gibraltar fever, Malta fever, Maltese fever, Mediterranean fever, rock fever, or undulant fever – is a zoonotic infection of domesticated and wild animals, caused by bacteria of the genus Brucella. Humans become infected by ingestion of food products of animal origin (such as undercooked meat or unpasteurized milk or dairy products), direct contact with infected animals, or inhalation of infectious aerosols. Transmission from human to human is rare but possible. Species infecting domestic livestock are B. melitensis (goats and sheep), B. suis (pigs), B. abortus (cattle and bison), B. ovis (sheep) and B. canis (dogs).

Description: BactoReal® Kit Brucella spp. is based on the amplification and detection of the IS711 region of B. abortus, B. ovis, B. melitensis, B. suis and B. canis using real-time PCR.

PCR-platforms: BactoReal® Kit Brucella spp. is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Brucella spp.


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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