BactoReal® Kit Chlamydophila psittaci/abortus

 

BactoReal Kit Chlamydophila psittaci_abortus

 

BactoReal®  Kit Chlamydophila psittaci/abortus

Order no.

Reactions

Pathogen

Internal positive control

DVEB03013

100

FAM channel

Cy5 channel

DVEB03053

50

FAM channel

Cy5 channel

DVEB03011

100

FAM channel

VIC/HEX channel

DVEB03051

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Chlamydophila psittaci and C. abortus
Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Chlamydophila psittaci
Water

Background: Chlamydophila psittaci (previously classified as Chlamydia psittaci) is an intracellular bacterium that may cause endemic avian chlamydiosis in feral birds and domesticated poultry, epizootic outbreaks in mammals such as cattle, pigs, sheep and horses, and respiratory psittacosis in humans (also known as parrot fever and ornithosis). It is transmitted by inhalation, contact or ingestion among birds and to mammals. Psittacosis in birds and in humans often starts with flu-like symptoms and becomes a life-threatening pneumonia. Chlamydophila psittaci is present in feces, nasal secretions, and feathers of infected birds. Chlamydophila abortus (previously classified as Chlamydia psittaci serotype 1) is one of the major infectious causes of abortion in sheep, cattle and goats worldwide by colonizing in the placenta. Enzootic abortion in sheep is also known as ovine enzootic abortion (OEA) or enzootic abortion of ewes (EAE). Infection with strains of this microorganism has also been associated with abortion and other clinical symptoms also in humans.

Description: BactoReal® Kit Chlamydophila psittaci/abortus is based on the amplification and detection of the omp1 gene of C. psittaci and C. abortus using real-time PCR.

PCR-platforms: BactoReal® Kit Chlamydophila psittaci/abortus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Chlamydophila psittaci/abortus


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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