BactoReal® Kit Clostridium perfringens

 

BactoReal Kit Clostridium perfringens

 

BactoReal® Kit Clostridium perfringens

Order no.

Reactions

Pathogen

Internal positive control

DVEB06513

100

FAM channel

Cy5 channel

DVEB06553

50

FAM channel

Cy5 channel

DVEB06511

100

FAM channel

VIC/HEX channel

DVEB06551

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for
Clostridium perfringens
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for C. perfringens
– Water

Background: Clostridium perfringens is a spore-forming gram-positive bacterium present in many environmental sources as well as in the intestines of humans and animals. Under certain predisposing conditions, Clostridium perfringens proliferates rapidly, produces toxins and causes disease such as gas gangrene, necrotic enteritis and enterotoxemia. This bacterium is an important cause of food poisoning. Stress and the feed composition are believed to be of major importance for the development of disease. Clostridium perfringens strains are classified into five types (A–E) on the basis of their ability to produce the major lethal toxins alpha (cpa), beta (cpb), epsilon (etx), and iota (iap) which give the cytotoxic and necrotic activity associated with infection. In addition, strains of C. perfringens may also produce a number of other toxins, including enterotoxin (cpe), beta-2 toxin (cpb2) or theta toxin.

Description: BactoReal® Kit Clostridium perfringens detects all five serotypes of C. perfringens and is based on the amplification and detection of the 16S rRNA gene of Clostridium perfringens using real-time PCR.

PCR-platforms: BactoReal® Kit Clostridium perfringens is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Clostridium perfringens


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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