BactoReal® Kit Corynebacterium pseudotuberculosis

 

BactoReal Kit Corynebacterium pseudotuberculosis

 

BactoReal® Kit Corynebacterium pseudotuberculosis
Order no. Reactions Pathogen

Internal positive control

DVEB05213

100

FAM channel

Cy5 channel

DVEB05253

50

FAM channel

Cy5 channel

DVEB05211

100

FAM channel

VIC/HEX channel

DVEB05251

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Corynebacterium pseudotuberculosis
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for C. pseudotuberculosis
– Water

Background: Corynebacterium pseudotuberculosis is a Gram-positive bacterium and the aetiological agent of external or cutaneous abscessation, lymphangitis, and internal abscessation affecting sheep, goats, horses, cattle and rarely humans as well. Corynebacterium pseudotuberculosis produces an exotoxin called phospholipase D that enhances dissemination of the bacteria by damaging endothelial cells and increasing vascular permeability.

Description: BactoReal® Kit Corynebacterium pseudotuberculosis is based on the amplification and detection of the phosholipase D (pld) gene of Corynebacterium pseudotuberculosis using real-time PCR.

PCR-platforms: BactoReal® Kit Corynebacterium pseudotuberculosis is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity: See product description

Also available as assay only: BactoReal® Corynebacterium pseudotuberculosis


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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