BactoReal® Kit E. coli Typing eae (EHEC or EPEC)

 

BactoReal Kit E coli Typing eae

 

 

Kit contents BactoReal® Kit E. coli Typing eae (EHEC or EPEC):
– Detection assay for E. coli virulence factor┬á
eae
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for┬áeae
– Water

Description: BactoReal® Kit E. coli Typing eae (EHEC or EPEC) is a real-time PCR kit for detection of the virulence-associated gene eae of EPEC (only positive for eae; negative for ehxA and stx1, stx2) and EHEC (positive for eae or ehxA; also positive for stx1 and/or stx2) . Eae is detected in FAM channel. This kit belongs to a series of different real-time PCR kits for detection of virulence-associated genes of E. coli (BactoReal® Kit E. coli Typing).

PCR-platforms: BactoReal® Kit E. coli Typing eae (EHEC or EPEC) is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assayversion: BactoReal® E. coli Typing eae (EHEC or EPEC)

Background: Escherichia coli is a gram negative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals.
Enteropathogenic Escherichia coli (EPEC) produces an outer membrane protein called intimin, encoded by eae and mediating intestinal cell attachment. Intimin is a virulence factor (adhesin) of EPEC and EHEC strains. Furthermore, EPEC are classified into typical and atypical strains based on the presence of the plasmid E. coli adherence factor (EAF). Typical EPEC strains are rarely isolated from animals, and humans are the major natural reservoir. The reservoir for atypical EPEC can be both animals and humans. By definition all EPEC lack genes to produce shiga toxin (stx).
Shiga toxin (verotoxin) producing Escherichia coli (STEC) is recognized as an important human diarrheal pathogen. Swine and cattle play an important role as a carrier of STEC. The ability of STEC to cause illness is due to the production of Shiga toxins Stx1 and/or Stx2 (encoded by stx1 and stx2 genes).
Enterohaemorrhagic Escherichia coli (EHEC) can cause severe foodborne disease. EHEC is also positive for stx1 and/or stx2 but also produces either intimin (eae) or hemolysin (ehxA).

Series BactoReal® Kit E. coli Typing
BactoReal® Kit Detection of Pathogen IPC Order no. Reactions
E. coli Typing stx1 & stx2 (STEC) stx1 & stx2 FAM + VIC/HEX Cy5 DVEB04013 100
DVEB04053 50
E. coli Typing eae & ehxA (EHEC, EPEC) eae & ehxA FAM + VIC/HEX Cy5 DVEB04113 100
DVEB04153 50
E. coli Typing eae (EHEC or EPEC) eae FAM Cy5 DVEB04313 100
DVEB04353 50
E. coli Typing ehxA (EHEC) ehxA VIC/HEX Cy5 DVEB04413 100
DVEB04453 50

 

BactoReal® Kit E. coli Typing is a series of different real-time PCR kits for detection of virulence-associated genes of E. coli. Depending on which kit and which combinations of kits are used, they allow the typing of enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and of Shiga toxin (verotoxin) producing E. coli (STEC).
Stx1 is detected in FAM channel. Stx2 is detected in VIC/HEX channel. Eae is detected in FAM channel. EhxA is detected in VIC/HEX channel. Assays for stx1 and stx2, as well as assays for eae and ehxA can be combined in a multiplex PCR.


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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