BactoReal® Kit Enterococcus spp.

 

BactoReal Kit Enterococcus spp

 

BactoReal® Kit Enterococcus spp.
Order no. Reactions Pathogen

Internal positive control

DVEB03413

100

FAM channel

Cy5 channel

DVEB03453

50

FAM channel

Cy5 channel

DVEB03411

100

FAM channel

VIC/HEX channel

DVEB03451

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Enterococcus species
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Enterococcus
– Water

Background: The genus Enterococcus includes more than 17 species, until 1984 Enterococcus species were classified as Group D Streptococcus. This genus belongs to lactic acid bacteria of the phylum Firmicutes. Enterococci are Gram-positive cocci, they are facultative anaerobic organisms that can survive and grow in many environments. They are part of the normal intestinal flora of humans and animals but have also been found in soil, water, plants and insects. Enterococci are important pathogens responsible for serious infections. In cattle, enterococci have been associated with diarrhea in calves and bovine mastitis in dairy cattle.

Description: BactoReal® Kit Enterococcus spp. is based on the amplification and detection of the 23S rRNA gene of species of the genus Enterococcus using real-time PCR.

PCR-platforms:
BactoReal® Kit Enterococcus spp. is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Enterococcus spp.


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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