BactoReal® Kit Escherichia coli

 

BactoReal Kit Escherichia coli

 

 

BactoReal® Kit Escherichia coli
Order no. Reactions Pathogen

Internal positive control

DVEB01913

100

FAM channel

Cy5 channel

DVEB01953

50

FAM channel

Cy5 channel

DVEB01911

100

FAM channel

VIC/HEX channel

DVEB01951

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Escherichia coli
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for Escherichia coli
Water

Background: Escherichia coli is a gram negative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. The dxs gene (1-deoxyxylulose-5-phosphate synthase gene) can be found in E. coli and Shigella strains.

Description: BactoReal® Kit Escherichia coli is based on the amplification and detection of the dxs gene of E. coli and Shigella using real-time PCR.

PCR-platforms: BactoReal® Kit Escherichia coli is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Escherichia coli


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

preloaderpreloaderpreloaderpreloader