BactoReal® Kit European Foulbrood

 

BactoReal Kit European Foulbrood

 

BactoReal® Kit European Foulbrood

Order no.

Reactions

Pathogen

Internal positive control

DVEB05513

100

FAM channel

Cy5 channel

DVEB05553

50

FAM channel

Cy5 channel

DVEB05511

100

FAM channel

VIC/HEX channel

DVEB05551

50

FAM channel

VIC/HEX channel

 

Kit content:
– Detection assay for┬áMelissococcus plutonius

– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for┬áMelissococcus plutonius
– Water

Background: European foulbrood (EFB) is a bacterial brood disease of honey bees caused by the Gram-positive bacterium Melissococcus plutonius. The disease occurs throughout the world. It may cause serious losses of brood. Larvae get infected by consuming contaminated food fed by the nurse bees. The bacteria multiply within the larval gut, competing with it for its food. Larvae that die from the disease have been starved of food.

Description: BactoReal® Kit European Foulbrood is based on the amplification and detection of the 16S rRNA gene of Melissococcus plutonius using real-time PCR.

PCR-platforms: BactoReal® Kit European Foulbrood is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® European Foulbrood


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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