BactoReal® Kit Haemophilus parasuis

 

BactoReal Kit Haemophilus parasuis

 

BactoReal®  Kit Haemophilus parasuis

Order no.

Reactions

Pathogen

Internal positive control

DVEB00413

100

FAM channel

Cy5 channel

DVEB00453

50

FAM channel

Cy5 channel

DVEB00411

100

FAM channel

VIC/HEX channel

DVEB00451

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Haemophilus parasuis
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for H. parasuis
Water

Background: Haemophilus parasuis is a Gram-negative bacterium (member of the Pasteurellaceae) of the upper respiratory tract of swine. It is the aetiological agent of Gl├Ąssers disease, a severe systemic disease characterized by fibrinous polyserositis, pericarditis, arthritis and meningitis. In addition to the acute form of the disease, Haemophilus parasuis is an important component of the Porcine Respiratory Disease Complex (PRDC). Fifteen serovars of H. parasuis have been reported, but a high percentage of the evaluated isolates are nontypeable. Isolates from the upper respiratory tract are mainly apathogenic strains of clinically healthy swine.

Description: BactoReal® Kit Haemophilus parasuis is based on the amplification and detection of the 16S rRNA gene of H. parasuis using real-time PCR.

PCR-platforms: BactoReal® Kit Haemophilus parasuis is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Haemophilus parasuis


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

preloaderpreloaderpreloaderpreloader